Diseño y estudio de compuestos de rutenio con actividad biológica
- Lenis Rojas, Óscar Armando
- Jesús José Fernández Sánchez Co-director
- Digna Vázquez Co-director
Defence university: Universidade da Coruña
Fecha de defensa: 22 December 2017
- Ignacio del Río Chair
- Alberto Ángel Férnandez López Secretary
- Alexandra Fernandes Committee member
Type: Thesis
Abstract
This project is based on the synthesis and structural characterization of new mono- and polinuclear coordination compounds of ruthenium derived from bipyridine and new mononuclear organometallic compounds of ruthenium derived from pcymene, and the study of their cytotoxic properties and in vivo toxicology in order to determine their applicability as antitumor agents. The structural determination has been carrie out by elemental analysis, mass spectrometry, 1H and 31P-{1H} NMR, UV-Vis and IR spectroscopy, conductivity measurements, cyclic voltammetry and single-crystal X-Ray difraction. It was evaluated the cytotoxic activity in human cancer cell lines, the interaction with DNA as target molecule and the interaction with albumin as transport system were evaluated. The in vivo toxicology was performed using the zebrafish model. The overall goal of the studies allowed to stablish the relationship between the structure and the behaviour of the compounds as antineoplastic agents. The results obtained results indicate that the compounds show a similar or even higher cytotoxic activity compared with cis-platinum, a minimum cytotoxicity against non-tumour cells as well as low or no lethality in the zebrafish model.