Diferenciación epitelial in vitro de células mesenquimales troncales humanas (hCMTs) de tejido adiposo (TA) y médula ósea (MO): caracterización celular y estudio de expresión de HLA I y II
- Sierra-Sánchez, Álvaro 1
- Ruíz-García, Antonio 1
- Guerrero Calvo, Jorge 1
- Fernández Porcel, Natividad 1
- Espinosa Ibáñez, Olga 1
- Lizana Moreno, Antonio Manuel 1
- Fernández González, Ana 1
- Aneiros-Fernández, José 1
- Arias-Santiago, Salvador 1
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1
Universidad de Granada
info
ISSN: 0365-7965
Ano de publicación: 2016
Tomo: 101
Número: 798
Páxinas: 72-78
Tipo: Artigo
Outras publicacións en: Actualidad médica
Resumo
Background: Human mesenchymal stem cells derived from adipose tissue and bone marrow are one of the most common cell therapy procedures used in recent clinical trials due to their immunomodulation capacity. Furthermore, for their epithelial differentiation potential can be used in tissue engineering, incorporated in artificial tissues such as skin and cornea, replacing autologous epithelial cells. It is necessary to make a correct cellular characterization of differentiated cells and to study the effect in HLA I and II expression. Objetives: Characterization and quality controls under GMP conditions of in vitro differentiated human mesenchymal stem cells from different sources (adipose tissue and bone marrow) to epithelial lineage, and study of HLA I and II expression before and after differentiation. Methods: Isolation and expansion of two human mesenchymal stem cells lines from their tissues of origin and in vitro differentiation to epithelial cells using culture mediums supplemented with specific growth factors. Quality controls according Good Manufacturing Practices have been made and HLA I and II expression before and after differentiation have been studied. Finally, characteristics of differentiated cells have been demonstrated by histological and immunohistochemical analysis. Results: Two human mesenchymal stem cells lines from adipose tissue and bone marrow have been isolated complying with the proposed quality controls. After in vitro differentiation, human mesenchymal stem cells do not express HLA (I and II) markers, which are important in immune response, but weakly express proteins related to main epithelial layers of human skin (CK5, CK6 and CK14). Conclusion: The absence of expression of HLA I and II by flow cytometry in differentiated cells would promote the use of them with allogenic character to construct human skin and cornea by tissue engineering, however, more studies and protocols are required to confirm these preliminary results and to optimize in vitro differentiation of human mesenchymal stem cells.