Optimización del cultivo de queratinocitos humanos para el desarrollo de un modelo de piel artificial humana: alternativas celulares como capa alimentadora

  1. Fernández González, Ana 1
  2. Lizana Moreno, Antonio Manuel 1
  3. De Pablos Ramos, María del Mar 1
  4. Ruíz García, Antonio 1
  5. Espinosa Ibáñez, Olga 1
  6. Fernández Porcel, Natividad 1
  7. Guerrero Calvo, Jorge 1
  8. Arrabal, Miguel 1
  9. López-Carmona, F. 1
  10. Arias-Santiago, Salvador 1
  1. 1 Universidad de Granada
    info

    Universidad de Granada

    Granada, España

    ROR https://ror.org/04njjy449

Revista:
Actualidad médica

ISSN: 0365-7965

Ano de publicación: 2016

Tomo: 101

Número: 798

Páxinas: 85-94

Tipo: Artigo

DOI: 10.15568/AM.2016.798.OR04 DIALNET GOOGLE SCHOLAR lock_openAcceso aberto editor

Outras publicacións en: Actualidad médica

Resumo

Purpose: This study aims to optimize keratinocyte culture to develop an artificial human skin model. For this purpose, human cells are used as feeder layer: human dermal fibroblasts and adipose derived mesenchymal stem cells. The results obtained are compared with 3T3 fibroblasts, murine feeder layer used for decades. Methods: We conducted an experimental study using human and murine sub-lethally irradiated cells as feeder layer for the establishment of keratinocyte culture. Cell expansion rate and doubling rate were evaluated in the keratinocyte cell passage and in the final cell recovery (was carried out at 3 weeks). The yield and viability of keratinocytes were also evaluated in the initial processing. Results: The results determine that irradiated human dermal fibroblasts and irradiated adipose derived mesenchymal stem cells can act as feeder layer promoting adhesion and expansion of keratinocytes. Human dermal fibroblasts provide comparable results to those obtained with murine 3T3 fibroblasts. Conclusions: Irradiated human dermal fibroblasts provide a functional feeder layer which allows effectively in vitro expansion of keratinocytes to be used for clinical purposes for the development of an artificial human skin model.

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Fonte de datos: Dialnet