Evaluación multicéntrica de distintos métodos para la determinación de colesterol HDL directo con respecto al colesterol HDL de precipitación
- Juan Antonio Gómez Gerique
- Beatriz Martín Ballesteros
- Romina García Sardina
- Margarita Esteban Salán
- Fernando Fabiani Romero
- José Puzo Foncillas
- José Ángel Aguilar Doreste
- Pilar Calmarza Calmarza
- Milagros Díez Vázquez
- Carlos Romero Román
ISSN: 1888-4008
Ano de publicación: 2012
Volume: 5
Número: 1
Páxinas: 18-27
Tipo: Artigo
Outras publicacións en: Revista del laboratorio clínico
Resumo
Introduction: The results of quality assurance programs suggest that there are significant differences between the homogeneous methods available for the measurement of HDL cholesterol. However, the possible influence of matrix effects in the lyophilized samples used and the absence of a true value in these samples means that a full assessment of these methods cannot be made. In this multicentre pilot study we compared the most used homogeneous methods in our country with the phosphotungstic acid-MgCl2 method. Material and methods: Each laboratory processed about one hundred serum samples from patients with one or two homogeneous methods and sent frozen aliquots of the samples in special packaging with dry ice to the central laboratory where they were processed by the precipitation method. Results: The imprecision of homogeneous methods were good and lower than those of the precipitation method, and met the quality specifications for bias and total error (TE). However, not all homogeneous methods met the minimum quality objectives, as some procedures produced a very high positive bias (relative to the comparison method) and one, a low negative bias. In addition, the study of the agreement between methods, made by stratifying patients according to HDL cholesterol concentrations, showed differences. Conclusions: These results suggest that there are discrepancies in the assessment of the levels obtained with homogeneous methods, probably due to a high inaccuracy in some of them, and it is advised that the laboratories be aware of the characteristics of inaccuracy and bias of the direct method used compared to the reference method.