Validez de un método fotométrico de medición de partículas en aire para el control de quirófanos y otras salas de ambiente controlado en los hospitales

  1. Berta Uriel Latorre
  2. D. Seoane Mato
  3. María Teresa Alves
  4. María Jesús Azuara García
  5. María Jesús García García
  6. A. Blanco Rodríguez
  7. Lisset Fernández de Armas
  8. Servando López Álvarez
  9. María Cruz Méndez Diéguez
Journal:
Trauma

ISSN: 1888-6116

Year of publication: 2013

Volume: 24

Issue: 3

Pages: 195-199

Type: Article

More publications in: Trauma

Abstract

Objective: Validate a photometric on-air particle measurement system in order to perform ambient bio-secu- rity checks in operating rooms and other clean rooms within hospitals. Material and methods: A total of 144 coupled air samples were performed, 88 in 8 different operating rooms with conventional ventilation systems and 3-level filtering and 56 in 9 different rooms without specific ventilation systems. Particles were measured in size between 0,1 to 10 ?m with the photometric system in mg/m 3 . Simultane- ously in the rooms are performed the sampling air method with impact on culture media. Incubation period is 5 days and count is performed in colony-forming units (CFU/1000L). Finally, optimal cut-points in particles mea- sure were calculated using ROC curves in order to discriminate the three different contamination levels. Results: Spearman�s correlation coefficient was 0,779 p<0,001. Sensitivity for cut-points in particle counting for the different contamination levels was 95,83% ,100% and 100% respectively, and the corresponding Kap- pa indexes were 0,51; 0,88 and 0,75. Conclusion: Correlation among measurements is notable. Particle measurement can be a worthy alternative for some of the routine bio-security checks