Rutas moleculares que determinan el posicionamiento de los centriolos en células de drosophila y la diferenciación del epitelio respiratorio de vertebrados

Abstract

Throughout the development of multicellular organisms, a multitude of processes that are closely regulated take place simultaneously, and in which the alteration of this regulation causes serious defects, affecting the viability of individuals. Among these processes, the positioning of organelles inside the cell or an adequate balance between the cell populations that make up different tissues play an essential role in development and morphogenesis. Specifically, the correct positioning of organelles such as centrioles is key in targeted cell division or coordinated movement of cilia. Previous studies demonstrated that the planar cell polarity pathway (Fz-PCP) plays an important role in establishing these positional patterns. In the present work we delve into this regulation through the study of the role of what are known as PCP effectors, as well as the role of the actin cytoskeleton in the regulation of centriole polarity. In addition, we have also studied the role of another PCP pathway known as Ft / Ds-PCP. On the other hand, cell signaling mediated by proteins such as p53 has proven to be key to the correct development of multicellular organisms. Members of the p53 family such as p73 and p63 play a key role in the differentiation of the respiratory epithelium, however, the role of p53 in this process is largely unknown. Based on this, we propose to characterize the role of P53 in the differentiation of the respiratory epithelium.